Saturation mutagenesis defines novel mouse models of severe spine deformity
نویسندگان
چکیده
منابع مشابه
Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution.
Sequence saturation mutagenesis (SeSaM) is a conceptually novel and practically simple method that truly randomizes a target sequence at every single nucleotide position. A SeSaM experiment can be accomplished within 2-3 days and comprises four steps: generating a pool of DNA fragments with random length, 'tailing' the DNA fragments with universal base using terminal transferase at 3'-termini, ...
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ژورنال
عنوان ژورنال: Disease Models & Mechanisms
سال: 2021
ISSN: 1754-8403,1754-8411
DOI: 10.1242/dmm.048901